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Doctoral-Level Mitosis: Molecular Mechanics and Regulation

Worksheet
Doctoral
English

Doctoral-Level Mitosis: Molecular Mechanics and Regulation

Activity 1: Molecular Sequencing of the Prophase-to-Metaphase Transition

This task requires you to arrange the biochemical and structural events that facilitate the transition from late prophase through the satisfaction of the Spindle Assembly Checkpoint (SAC). Focus on the precise choreography of the nuclear envelope breakdown (NEBD), the assembly of the outer kinetochore, and the signaling cascades of the SAC.
fig 1: Conceptual illustration of the kinetochore-microtubule interface, highlighting the molecular complexity of the KMN network and inner centromere signaling.
fig 1: Conceptual illustration of the kinetochore-microtubule interface, highlighting the molecular complexity of the KMN network and inner centromere signaling.

Instructions: Order the following 10 events chronologically (1–10). Write the corresponding letter in the space provided.
Randomized Events:
  • A. Sequestration of Cdc20 by the Mitotic Checkpoint Complex (MCC).
  • B. Cdk1/Cyclin B1-mediated phosphorylation of Lamins A, B, and C (specifically at Ser22 and Ser392).
  • C. Dissociation of the MCC and release of Cdc20 upon achieving amphitelic (bipolar) orientation.
  • D. Recruitment of the KMN network (KNL1 complex, Mis12 complex, and Ndc80 complex) to the kinetochore.
  • E. Mps1-mediated phosphorylation of KNL1 "MELT" repeats on unattached kinetochores.
  • F. Stabilization of the CCAN (Constitutive Centromere-Associated Network) on CENP-A nucleosomes.
  • G. Retraction of the nuclear membrane into the endoplasmic reticulum (ER) following lamina disassembly.
  • H. Aurora B kinase-dependent phosphorylation of the Ndc80 N-terminal tail to resolve syntelic or merotelic attachments.
  • I. Plk1-mediated phosphorylation of nucleoporins (Nup98 and the Nup107-160 complex).
  • J. Loading of the Ndc80 complex onto spindle microtubules to form the primary load-bearing attachment.
Your Sequence:
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